Tg(Ltf-icre)14Mmul
Transgene Detail

Summary

• Symbol: Tg(Ltf-icre)14Mmul
• Name: transgene insertion 14, Mathias Muller
• MGI ID: MGI:5571915
• Synonyms: Ltf-Cre
• Transgene: Tg(Ltf-icre)14Mmul Location: unknown
• Alliance: Tg(Ltf-icre)14Mmul page

Transgene origin

• Strain of Origin: (C57BL/6 x 129/Sv)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Ltf-icre)14Mmul expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Ltf

• Mutation details: A BAC (RP24-166N8) containing the Ltf gene was modified in bacterial cells to produce the targeting construct. A cassette containing codon-improved cre recombinase (icre), an artificial intron, the bovine growth hormone polyadenylation signal and an frt-flanked ampicillin selection marker was inserted into the first exon of Ltf. A plasmid endoding Flp was transfected into E. coli cells to delete the amp-marker. The BAC construct was verified by sequencing and microinjected into pronuclei of (B6/129Sv)F1 oocytes to produce transgenic animals. 2 lines (14 and 15) were obtained. Line 14 was chosen for further analysis. (J:210800)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ltf (species not specified) Summary of all recombinase alleles driven by Ltf.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:210800 Kovacic B, et al., Lactotransferrin-Cre reporter mice trace neutrophils, monocytes/macrophages and distinct subtypes of dendritic cells. Haematologica. 2014 Jun;99(6):1006-15
• All: 4 reference(s)