Rapgef4^tm1.1Chen
Targeted Allele Detail

Summary

• Symbol: Rapgef4^tm1.1Chen
• Name: Rap guanine nucleotide exchange factor (GEF) 4; targeted mutation 1.1, Ju Chen
• MGI ID: MGI:5571844
• Synonyms: Epac2^flox
• Gene: Rapgef4 Location: Chr2:71811584-72087818 bp, + strand Genetic Position: Chr2, 42.9 cM, cytoband C3
• Alliance: Rapgef4^tm1.1Chen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210426
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 9. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 9. Flp-mediated recombination removed the selection cassette. (J:210426)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rapgef4 Mutation: 66 strains or lines available

Notes

The targeted exon was initially unclear J:210426, but it has been confirmed as exon 9 (not 7) by the allele developer.

References

• Original: J:210426 Pereira L, et al., Epac2 mediates cardiac beta1-adrenergic-dependent sarcoplasmic reticulum Ca2+ leak and arrhythmia. Circulation. 2013 Feb 26;127(8):913-22
• All: 2 reference(s)