Fads3^{tm1e(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Fads3^{tm1e(EUCOMM)Hmgu}
• Name: fatty acid desaturase 3; targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5568454
• Gene: Fads3 Location: Chr19:10018933-10037474 bp, + strand Genetic Position: Chr19, 6.33 cM, cytoband B
• IMPC: Fads3 gene page

Mutation origin

• Mutant Cell Lines: HEPD0959_5_A01, HEPD0959_5_A02, HEPD0959_5_A03, HEPD0959_5_A04, HEPD0959_5_A05, HEPD0959_5_A06, HEPD0959_5_A08, HEPD0959_5_A09, HEPD0959_5_A11, HEPD0959_5_A12, HEPD0959_5_B01, HEPD0959_5_B03, HEPD0959_5_B04, HEPD0959_5_B05, HEPD0959_5_B06, HEPD0959_5_B07, HEPD0959_5_B09, HEPD0959_5_B10, HEPD0959_5_B11, HEPD0959_5_B12, HEPD0959_5_C01, HEPD0959_5_C02, HEPD0959_5_C03, HEPD0959_5_C05, HEPD0959_5_C07, HEPD0959_5_C08, HEPD0959_5_C09, HEPD0959_5_C11, HEPD0959_5_C12, HEPD0959_5_D01, HEPD0959_5_D02, HEPD0959_5_D03, HEPD0959_5_D06, HEPD0959_5_D07, HEPD0959_5_D08, HEPD0959_5_D09, HEPD0959_5_D10, HEPD0959_5_D11, HEPD0959_5_D12, HEPD0959_5_E01, HEPD0959_5_E02, HEPD0959_5_E03, HEPD0959_5_E04, HEPD0959_5_E06, HEPD0959_5_E07, HEPD0959_5_E08, HEPD0959_5_E09, HEPD0959_5_E10, HEPD0959_5_E11, HEPD0959_5_E12, HEPD0959_5_F01, HEPD0959_5_F03, HEPD0959_5_F04, HEPD0959_5_F05, HEPD0959_5_F06, HEPD0959_5_F07, HEPD0959_5_F08, HEPD0959_5_G01, HEPD0959_5_G03, HEPD0959_5_G04, HEPD0959_5_G05, HEPD0959_5_G06, HEPD0959_5_G07, HEPD0959_5_G08, HEPD0959_5_G10, HEPD0959_5_G11, HEPD0959_5_G12, HEPD0959_5_H01, HEPD0959_5_H02, HEPD0959_5_H03, HEPD0959_5_H04, HEPD0959_5_H05, HEPD0959_5_H06, HEPD0959_5_H07, HEPD0959_5_H10, HEPD0959_5_H12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 10029205 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fads3 Mutation: 21 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)