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Hprttm366a(Ple67-icre/ERT2)Ems
Targeted Allele Detail
Nomenclature
Symbol: Hprttm366a(Ple67-icre/ERT2)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 366a, Elizabeth M Simpson
MGI ID: MGI:5568249
Synonyms: FEV-creERT2, mEMS6017, Ple67-icre/frt/ERT2/frt
Gene: Hprt  Location: ChrX:52988137-53021659 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Line:  mEMS6017
Germline Transmission:  Earliest citation of germline transmission: J:204202
Parent Cell Line:  mEMS4855.02 (ES Cell)
Strain of Origin:  B6N.Cg-Aw-J Hprtb-m3
Project Collection: EUCOMMTOOLS
Mutation
description
Allele Type:    Targeted (Conditional ready, Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mutation detailsThe transgene was designed with an HS4 insulator region, the 2202 bp Ple67 minipromoter (derived from a portion of the endogenous promoter (Long Prom) from the human FEV (ETS oncogene family)), a chimeric intron cassette, the iCre/ERT2 fusion gene (with F3-frt sites flanking the ERT2 sequences), the mut6-WPRE element, an SV40 early polyA signal, an additional HS4 insulator region, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. (J:270567)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: FEV     Summary of all recombinase alleles driven by FEV.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1272 strains or lines available
References
Original:  J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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Funding Information
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last database update
04/06/2021
MGI 6.16
The Jackson Laboratory