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Cyp4f18tm1.1Pchr
Targeted Allele Detail
Nomenclature
Symbol: Cyp4f18tm1.1Pchr
Name: cytochrome P450, family 4, subfamily f, polypeptide 18; targeted mutation 1.1, Peter Christmas
MGI ID: MGI:5565359
Synonyms: Cyp4f18-, Cyp4f18 knockout
Gene: Cyp4f18  Location: Chr8:71988482-72009626 bp, - strand  Genetic Position: Chr8, 34.67 cM, cytoband C1
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:212489
Parent Cell Line:  D1 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6J)F1
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsFlp recombinase expression was used to excise an FRT flanked neo cassette. Subsequent expression of cre removed the loxP flanked exons 8 and 9. Real time PCR analysis confirmed loss of exon 8 and 9 expression in homozygous mutant mice. The mutant RNA transcript codes for a truncated translation product that lacks the C-terminal 219 amino acids, because deletion of exons 8 and 9 results in a frameshift mutation and introduces a premature stop codon 40 bp downstream of the start of exon 10. Western blot analysis of membrane protein extracts from mutant neutrophils using an antibody that reacts with amino acids 353-370 confirmed loss of expression of this critical C-terminal region. (J:212489)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cyp4f18 Mutation:  24 strains or lines available
References
Original:  J:212489 Winslow V, et al., Altered leukotriene B4 metabolism in CYP4F18-deficient mice does not impact inflammation following renal ischemia. Biochim Biophys Acta. 2014 Jun;1841(6):868-79
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/07/2017
MGI 6.11
The Jackson Laboratory