Cyp4f18^tm1.1Pchr
Targeted Allele Detail

Summary

• Symbol: Cyp4f18^tm1.1Pchr
• Name: cytochrome P450, family 4, subfamily f, polypeptide 18; targeted mutation 1.1, Peter Christmas
• MGI ID: MGI:5565359
• Synonyms: Cyp4f18^-, Cyp4f18 knockout
• Gene: Cyp4f18 Location: Chr8:72742326-72763470 bp, - strand Genetic Position: Chr8, 34.67 cM, cytoband C1
• Alliance: Cyp4f18^tm1.1Pchr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212489
• Parent Cell Line: D1 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Flp recombinase expression was used to excise an FRT flanked neo cassette. Subsequent expression of cre removed the loxP flanked exons 8 and 9. Real time PCR analysis confirmed loss of exon 8 and 9 expression in homozygous mutant mice. The mutant RNA transcript codes for a truncated translation product that lacks the C-terminal 219 amino acids, because deletion of exons 8 and 9 results in a frameshift mutation and introduces a premature stop codon 40 bp downstream of the start of exon 10. Western blot analysis of membrane protein extracts from mutant neutrophils using an antibody that reacts with amino acids 353-370 confirmed loss of expression of this critical C-terminal region. (J:212489)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp4f18 Mutation: 38 strains or lines available

References

• Original: J:212489 Winslow V, et al., Altered leukotriene B4 metabolism in CYP4F18-deficient mice does not impact inflammation following renal ischemia. Biochim Biophys Acta. 2014 Jun;1841(6):868-79
• All: 3 reference(s)