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Spp2tm1(cre/ERT2)Amc
Targeted Allele Detail
Nomenclature
Symbol: Spp2tm1(cre/ERT2)Amc
Name: secreted phosphoprotein 2; targeted mutation 1, Andrew P McMahon
MGI ID: MGI:5565344
Synonyms: Spp2G2aCE, Spp2tm1(GFP,cre/ERT2)Amc
Gene: Spp2  Location: Chr1:88406961-88426438 bp, + strand  Genetic Position: Chr1, 44.63 cM, cytoband C5
Mutation
origin
Mutant Cell Line:  EPD0558_1_A04
Germline Transmission:  Earliest citation of germline transmission: J:159099
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Mutation
description
Allele Type:    Targeted (Inducible, Null/knockout, RMCE-ready, Recombinase, Reporter)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe allele was generated by a dual recombinase-mediated cassette exchange protocol. The exchange vector contained (from 5' to 3') an frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) sequence, a viral T2A oligopeptide, and a cre/ERT2 sequence. A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector. (J:159099)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Spp2     Summary of all recombinase alleles driven by Spp2.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Spp2 Mutation:  16 strains or lines available
Notes
This exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-Atm1Brd-derived JM8A3.N1 knockout first reporter embryonic stem (ES) cells obtained from the European Conditional Mouse Mutagenesis (EUCOMM) consortium containing clone EPDO558_1_A04). Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony.
References
Original:  J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/15/2019
MGI 6.14
The Jackson Laboratory