Entpd5^{tm1(cre/ERT2)Amc}
Targeted Allele Detail

Summary

• Symbol: Entpd5^{tm1(cre/ERT2)Amc}
• Name: ectonucleoside triphosphate diphosphohydrolase 5; targeted mutation 1, Andrew P McMahon
• MGI ID: MGI:5565343
• Synonyms: Entpd5^G2aCE, Entpd5^{tm1(GFP,cre/ERT2)Amc}
• Gene: Entpd5 Location: Chr12:84420649-84455803 bp, - strand Genetic Position: Chr12, 39.18 cM, cytoband E
• Alliance: Entpd5^{tm1(cre/ERT2)Amc} page

Mutation origin

• Mutant Cell Line: EPD0112_5_E02
• Germline Transmission: Earliest citation of germline transmission: J:159099
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, RMCE-ready, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Entpd5^{tm1(cre/ERT2)Amc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Entpd5 (MGI:1321385)

• Mutation details: The allele was generated using a dual recombinase-mediated cassette exchange protocol. The exchange vector contained (from 5' to 3') an frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) seqe, a viral T2A oligopeptide, and a cre/ERT2 sequence. A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector. (J:159099)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Entpd5 (mouse) Summary of all recombinase alleles driven by Entpd5. Alliance mouse Entpd5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Entpd5 Mutation: 26 strains or lines available

Notes

This exchange vector (along with the pDIRE plasmid containing an icre-expressioncassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-derived JM8.F6 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone EPDO112_5_E02. Recombined ES cells, with the neo cassette removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony.

References

• Original: J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010
• All: 1 reference(s)