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Entpd5tm1(cre/ERT2)Amc
Targeted Allele Detail
Nomenclature
Symbol: Entpd5tm1(cre/ERT2)Amc
Name: ectonucleoside triphosphate diphosphohydrolase 5; targeted mutation 1, Andrew P McMahon
MGI ID: MGI:5565343
Synonyms: Entpd5G2aCE, Entpd5tm1(GFP,cre/ERT2)Amc
Gene: Entpd5  Location: Chr12:84420649-84455803 bp, - strand  Genetic Position: Chr12, 39.18 cM, cytoband E
Mutation
origin
Mutant Cell Line:  EPD0112_5_E02
Germline Transmission:  Earliest citation of germline transmission: J:159099
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Mutation
description
Allele Type:    Targeted (Inducible, Null/knockout, RMCE-ready, Recombinase, Reporter)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe allele was generated using a dual recombinase-mediated cassette exchange protocol. The exchange vector contained (from 5' to 3') an frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) seqe, a viral T2A oligopeptide, and a cre/ERT2 sequence. A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector. (J:159099)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Entpd5     Summary of all recombinase alleles driven by Entpd5.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Entpd5 Mutation:  21 strains or lines available
Notes
This exchange vector (along with the pDIRE plasmid containing an icre-expressioncassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-derived JM8.F6 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone EPDO112_5_E02. Recombined ES cells, with the neo cassette removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony.
References
Original:  J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory