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Targeted Allele Detail
Symbol: Entpd5tm1(cre/ERT2)Amc
Name: ectonucleoside triphosphate diphosphohydrolase 5; targeted mutation 1, Andrew P McMahon
MGI ID: MGI:5565343
Synonyms: Entpd5G2aCE, Entpd5tm1(GFP,cre/ERT2)Amc
Gene: Entpd5  Location: Chr12:84420649-84455803 bp, - strand  Genetic Position: Chr12, 39.18 cM, cytoband E
Mutant Cell Line:  EPD0112_5_E02
Germline Transmission:  Earliest citation of germline transmission: J:159099
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Allele Type:    Targeted (Inducible, Null/knockout, RMCE-ready, Recombinase, Reporter)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
Mutation detailsThe allele was generated using a dual recombinase-mediated cassette exchange protocol. The exchange vector contained (from 5' to 3') an frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) seqe, a viral T2A oligopeptide, and a cre/ERT2 sequence. A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector. (J:159099)
 Tissue activity of this recombinase allele
Driver: Entpd5     Summary of all recombinase alleles driven by Entpd5.

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Entpd5 Mutation:  21 strains or lines available
This exchange vector (along with the pDIRE plasmid containing an icre-expressioncassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-derived JM8.F6 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone EPDO112_5_E02. Recombined ES cells, with the neo cassette removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony.
Original:  J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory