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Tg(Prnp)E1Rchi
Transgene Detail
Nomenclature
Symbol: Tg(Prnp)E1Rchi
Name: transgene insertion E1, Roberto Chiesa
MGI ID: MGI:5563239
Synonyms: Tg(WT-E1)
Transgene: Tg(Prnp)E1Rchi  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6J x CBA/J)F2
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Prnp)E1Rchi expresses 1 gene
 
Mutation detailsThe transgene consists of 12 kb of the mouse Prnp promoter region, which includes intron 1, joined to a wild-type mouse Prnp cDNA. The transgene also includes an epitope tag for monoclonal antibody 3F4. Hemizygous mice of line E1 carry 9 copies of the transgene construct, as determined by quantitative Southern blot analysis. Western blot analysis of brain homogenates using the 3F4 antibody demonstrates expression of the mutant prion protein at 1.8-fold the level of endogenous hamster PrP. (J:51891)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:51891 Chiesa R, et al., Neurological illness in transgenic mice expressing a prion protein with an insertional mutation. Neuron. 1998 Dec;21(6):1339-51
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/05/2019
MGI 6.14
The Jackson Laboratory