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Targeted Allele Detail
Symbol: Pdyntm1.1(cre)Mjkr
Name: prodynorphin; targeted mutation 1.1, Michael J Krashes
MGI ID: MGI:5562873
Synonyms: Dyn-Cre, Pdyn-IRES-Cre, Prodynorphin-cre
Gene: Pdyn  Location: Chr2:129528485-129541764 bp, - strand  Genetic Position: Chr2, 63.19 cM
Germline Transmission:  Earliest citation of germline transmission: J:207472
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
Mutation detailsUsing recombineering methods, a selection cassette containing an internal ribosomal entry sequence (IRES) linked to cre and an frt-flanked kanamycin gene was inserted immediately downstream of the stop codon in exon 2 of the Pdyn gene in a BAC. This construct was used to isolate a targeting plasmid with the cre-kanamycin cassette with upstream and downstream Pdyn homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals. (J:207472)
 Tissue activity of this recombinase allele
Driver: Pdyn     Summary of all recombinase alleles driven by Pdyn. Comparative matrix view of recombinase activities

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pdyn Mutation:  14 strains or lines available
Original:  J:207472 Krashes MJ, et al., An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature. 2014 Mar 13;507(7491):238-42
All:  27 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory