Scrib^tm1.2Cj
Targeted Allele Detail

Summary

• Symbol: Scrib^tm1.2Cj
• Name: scribbled planar cell polarity; targeted mutation 1.2, Neal Copeland and Nancy Jenkins
• MGI ID: MGI:5559570
• Gene: Scrib Location: Chr15:75919011-75941633 bp, - strand Genetic Position: Chr15, 35.29 cM
• Alliance: Scrib^tm1.2Cj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204591
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 8. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 2 through 8. Western blot analysis confirmed the absence of protein expression in the brain. (J:204591)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scrib Mutation: 53 strains or lines available

References

• Original: J:204591 Yamben IF, et al., Scrib is required for epithelial cell identity and prevents epithelial to mesenchymal transition in the mouse. Dev Biol. 2013 Dec 1;384(1):41-52
• All: 2 reference(s)