Prdm4^{tm1a(EUCOMM)Liz}
Targeted Allele Detail

Summary

• Symbol: Prdm4^{tm1a(EUCOMM)Liz}
• Name: PR domain containing 4; targeted mutation 1a, Elizabeth K Bikoff
• MGI ID: MGI:5547611
• Synonyms: Prdm4^TA
• Gene: Prdm4 Location: Chr10:85727828-85752958 bp, - strand Genetic Position: Chr10, 41.75 cM
• Alliance: Prdm4^{tm1a(EUCOMM)Liz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205013
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The EUCOMM targeting vector (project ID 45696) introduced an FRT site, lacZ gene, loxP site, neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site upstream of exon 6. A third loxP site was inserted downstream of exon 7. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:205013)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm4 Mutation: 41 strains or lines available

References

• Original: J:205013 Bogani D, et al., The PR/SET domain zinc finger protein Prdm4 regulates gene expression in embryonic stem cells but plays a nonessential role in the developing mouse embryo. Mol Cell Biol. 2013 Oct;33(19):3936-50
• All: 1 reference(s)