Sox9^tm2.1Haak
Targeted Allele Detail

Summary

• Symbol: Sox9^tm2.1Haak
• Name: SRY (sex determining region Y)-box 9; targeted mutation 2.1, Haruhiko Akiyama
• MGI ID: MGI:5546592
• Synonyms: Sox9-EGFP
• Gene: Sox9 Location: Chr11:112673050-112678586 bp, + strand Genetic Position: Chr11, 77.27 cM
• Alliance: Sox9^tm2.1Haak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205660
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An IRES-EGFP-polyadenylation sequence and floxed neomycin resistance cassette were inserted into the first exon. Cre-mediated recombination removed the neomycin resistance cassette. RT-PCR confirmed the absence of transcript expression in EGFP+ cells. (J:205660)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox9 Mutation: 33 strains or lines available

References

• Original: J:205660 Nakamura Y, et al., Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25. Nat Commun. 2011;2:251
• All: 6 reference(s)