Usp13^{tm1e(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Usp13^{tm1e(EUCOMM)Hmgu}
• Name: ubiquitin specific peptidase 13 (isopeptidase T-3); targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5544955
• Gene: Usp13 Location: Chr3:32871695-32992220 bp, + strand Genetic Position: Chr3, 15.88 cM
• IMPC: Usp13 gene page

Mutation origin

• Mutant Cell Lines: HEPD0956_3_A05, HEPD0956_3_A12, HEPD0956_3_B03, HEPD0956_3_B04, HEPD0956_3_B05, HEPD0956_3_B06, HEPD0956_3_B09, HEPD0956_3_B11, HEPD0956_3_B12, HEPD0956_3_C03, HEPD0956_3_C04, HEPD0956_3_C10, HEPD0956_3_D03, HEPD0956_3_D07, HEPD0956_3_E01, HEPD0956_3_E05, HEPD0956_3_E07, HEPD0956_3_E11, HEPD0956_3_E12, HEPD0956_3_F05, HEPD0956_3_F09, HEPD0956_3_F10, HEPD0956_3_F11, HEPD0956_3_G01, HEPD0956_3_G12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_GT1_LF2A_LacZ_BetactP_neo
• Mutation details: The L1L2_GT1_LF2A_LacZ_BetactP_neo cassette was inserted at position 32930623 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Usp13 Mutation: 45 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)