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Sirt1tm1.1Vlcg
Targeted Allele Detail
Nomenclature
Symbol: Sirt1tm1.1Vlcg
Name: sirtuin 1; targeted mutation 1.1, Velocigene
MGI ID: MGI:5544792
Gene: Sirt1  Location: Chr10:63319005-63339035 bp, - strand  Genetic Position: Chr10, 32.57 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:200671
Parent Cell Line:  F1H4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NTac)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into exon 3 via homologous recombination, splitting exon 3 into 2 new exons (141 and 101 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the drug selection cassette. (J:200671)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Sirt1 Mutation:  51 strains or lines available
References
Original:  J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/26/2019
MGI 6.14
The Jackson Laboratory