Gt(ROSA)26Sor^tm2Vlcg
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm2Vlcg
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Velocigene
• MGI ID: MGI:5544780
• Synonyms: Gt(ROSA26)Sor^{i1COIN(dsRED2eGFP)}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm2Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200671
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 followed by a dsRed was inserted in the antisense orientation into intron 1 via homologous recombination, splitting intron 1 into 2 new exons (1,036 and 4,595 bp in length). A 2 bp deletion occurs at the site of insertion. The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, a neomycin resistance cassette was inserted after the the dsRed. (J:200671)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
• All: 1 reference(s)