Plekha8^tm1.2Aha
Targeted Allele Detail

Summary

• Symbol: Plekha8^tm1.2Aha
• Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8; targeted mutation 1.2, Akihiro Harada
• MGI ID: MGI:5544728
• Gene: Plekha8 Location: Chr6:54572096-54622824 bp, + strand Genetic Position: Chr6, 27.02 cM
• Alliance: Plekha8^tm1.2Aha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205015
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 4. An FRT-flanked splice acceptor IRES beta-geo polyadenylation sequence with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the beta-geo cassette and left exon 4 floxed. Cre-mediated recombination removed exon 4. Western blot analysis confirmed the absence of protein expression in testes and kidney. (J:205015)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plekha8 Mutation: 32 strains or lines available

References

• Original: J:205015 D'Angelo G, et al., Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi. Nature. 2013 Sep 5;501(7465):116-20
• All: 1 reference(s)