Grm5^tm1.1Pfw
Targeted Allele Detail

Summary

• Symbol: Grm5^tm1.1Pfw
• Name: glutamate receptor, metabotropic 5; targeted mutation 1.1, Paul F Worley
• MGI ID: MGI:5543408
• Synonyms: GRM5^AA, mGluR5^AA
• Gene: Grm5 Location: Chr7:87233376-87784115 bp, + strand Genetic Position: Chr7, 49.01 cM
• Alliance: Grm5^tm1.1Pfw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200071
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Two point mutations (A>G and T>G) were introduced in exon 8. A loxP site flanked neomycin resistance gene cassette was inserted into intron 7. The neo cassette was removed through subsequent cre-mediated recombination. The two mutations change a threonine and serine codon to alanine (p.Thr1123Ala and p.Ser1126Ala). This prevents phosphorylization of those two residues in the encoded protein. (J:200071)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grm5 Mutation: 79 strains or lines available

References

• Original: J:200071 Park JM, et al., A prolyl-isomerase mediates dopamine-dependent plasticity and cocaine motor sensitization. Cell. 2013 Aug 1;154(3):637-50
• All: 3 reference(s)