Pms2tm2(cre)Lisk
Targeted Allele Detail
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Symbol: |
Pms2tm2(cre)Lisk |
Name: |
PMS1 homolog2, mismatch repair system component; targeted mutation 2, Michael Liskay |
MGI ID: |
MGI:5538273 |
Synonyms: |
Pms2cre, Pms2Cre |
Gene: |
Pms2 Location: Chr5:143846782-143870786 bp, + strand Genetic Position: Chr5, 82.82 cM, cytoband G2
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Alliance: |
Pms2tm2(cre)Lisk page
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Germline Transmission: |
Earliest citation of germline transmission:
J:204653
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (Recombinase) |
Mutations: |
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Insertion, Intragenic deletion
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Pms2tm2(cre)Lisk expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The SV40 nuclear localization signal (NLS) within the cre sequence was modified to contain an 12-base pair A-T run which preserved the NLS, but added an extra A inducing a +1 bp shift. An IRES was cloned 5' to the cre ATG signal with a neo cassette cloned 3' to the recombinase sequence. The IRES-cre-neo vector was used for homologous recombination in ES cells, resulting in the IRES-cre inserting into the Pms2 locus replacing exon 2. The extra A in the NLS causes the cre to be out of frame (inactive). Because this Pms2 allele is null (thus deficient in DNA mismatch repair), homozygotes will have an increased frequency of -1 bp resulting in cre reversion and thus in cre expression.
(J:204653)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Pms2
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Pms2 Mutation: |
50 strains or lines available
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Original: |
J:204653 Miller AJ, et al., Tractable Cre-lox system for stochastic alteration of genes in mice. Nat Methods. 2008 Mar;5(3):227-9 |
All: |
5 reference(s) |
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