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Cd68tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Cd68tm1a(EUCOMM)Hmgu
Name: CD68 antigen; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:5532954
Gene: Cd68  Location: Chr11:69664213-69666153 bp, - strand  Genetic Position: Chr11, 42.86 cM
Mutation
origin
Mutant Cell Lines:  HEPD0946_1_A05, HEPD0946_1_A08
Germline Transmission:  Unknown
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: Ifitm2_intron_L1L2_GT2_LF2A_LacZ_BetactP_neo
 
Mutation detailsThe Ifitm2_intron_L1L2_GT2_LF2A_LacZ_BetactP_neo cassette was inserted at position 69665825 of Chromosome 11 (Build GRCm38) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 69664158. The critical artificial exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:157065)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Cd68 Mutation:  26 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/18/2020
MGI 6.14
The Jackson Laboratory