Clic4^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Clic4^{tm1a(EUCOMM)Hmgu}
• Name: chloride intracellular channel 4; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5532950
• Gene: Clic4 Location: Chr4:134941280-135000071 bp, - strand Genetic Position: Chr4, 67.19 cM, cytoband D3
• Alliance: Clic4^{tm1a(EUCOMM)Hmgu} page
• IMPC: Clic4 gene page

Mutation origin

• Mutant Cell Lines: HEPD0948_2_A03, HEPD0948_2_A05, HEPD0948_2_A06, HEPD0948_2_A07, HEPD0948_2_A08, HEPD0948_2_A09, HEPD0948_2_A10, HEPD0948_2_A11, HEPD0948_2_A12, HEPD0948_2_B01, HEPD0948_2_B02, HEPD0948_2_B04, HEPD0948_2_B05, HEPD0948_2_B07, HEPD0948_2_B08, HEPD0948_2_B09, HEPD0948_2_B10, HEPD0948_2_B11, HEPD0948_2_B12, HEPD0948_2_C02, HEPD0948_2_C03, HEPD0948_2_C04, HEPD0948_2_C05, HEPD0948_2_C06, HEPD0948_2_C07, HEPD0948_2_C08, HEPD0948_2_C09, HEPD0948_2_C10, HEPD0948_2_C11, HEPD0948_2_C12, HEPD0948_2_D01, HEPD0948_2_D05, HEPD0948_2_D06, HEPD0948_2_D08, HEPD0948_2_D09, HEPD0948_2_D10, HEPD0948_2_D11, HEPD0948_2_D12, HEPD0948_2_E01, HEPD0948_2_E02, HEPD0948_2_E03, HEPD0948_2_E04, HEPD0948_2_E05, HEPD0948_2_E06, HEPD0948_2_E07, HEPD0948_2_E09, HEPD0948_2_E10, HEPD0948_2_E11, HEPD0948_2_E12, HEPD0948_2_F01, HEPD0948_2_F02, HEPD0948_2_F03, HEPD0948_2_F04, HEPD0948_2_F05, HEPD0948_2_F06, HEPD0948_2_F07, HEPD0948_2_F08, HEPD0948_2_F10, HEPD0948_2_F11, HEPD0948_2_F12, HEPD0948_2_G01, HEPD0948_2_G02, HEPD0948_2_G03, HEPD0948_2_G04, HEPD0948_2_G05, HEPD0948_2_G06, HEPD0948_2_G08, HEPD0948_2_G09, HEPD0948_2_G10, HEPD0948_2_G11, HEPD0948_2_G12, HEPD0948_2_H01, HEPD0948_2_H02, HEPD0948_2_H03, HEPD0948_2_H04, HEPD0948_2_H05, HEPD0948_2_H07, HEPD0948_2_H08, HEPD0948_2_H09, HEPD0948_2_H11, HEPD0948_2_H12
• Germline Transmission: Earliest citation of germline transmission: J:339451
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 134965992 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 134966606. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clic4 Mutation: 39 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)