About   Help   FAQ
Hprttm340(Ple252-icre/ERT2)Ems
Targeted Allele Detail
Nomenclature
Symbol: Hprttm340(Ple252-icre/ERT2)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 340, Elizabeth M Simpson
MGI ID: MGI:5532387
Synonyms: Ple252-icre/ERT2;mEMS5708, SLITRK6-creERT2
Gene: Hprt  Location: ChrX:52988137-53021659 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Line:  mEMS5708
Germline Transmission:  Earliest citation of germline transmission: J:204202
Parent Cell Line:  mEMS4857.02 (ES Cell)
Strain of Origin:  B6N.Cg-Aw-J Hprtb-m3
Project Collection: EUCOMMTOOLS
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mutation detailsThe transgene consists of a RP11-398.A22 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene, an intron, an SV40 early polyA signal and a 48-bp full Frt (Ffrt) site-flanked kanamycin resistance (Kan) cassette were inserted directly at the ATG of the human SLITRK6 locus. Transient transfection with a FLP recombinase plasmid removed the Kan cassette before the modified BAC was used for targeting as a single copy "knock-in" into the upstream region of the Hprt locus. (J:270567)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: SLITRK6     Summary of all recombinase alleles driven by SLITRK6.
 

Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1272 strains or lines available
References
Original:  J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
04/06/2021
MGI 6.16
The Jackson Laboratory