Cfap298^{tm2a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cfap298^{tm2a(EUCOMM)Wtsi}
• Name: cilia and flagella associate protien 298; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:5527594
• Gene: Cfap298 Location: Chr16:90722697-90732002 bp, - strand Genetic Position: Chr16, 52.17 cM, cytoband C3.3
• IMPC: Cfap298 gene page

Mutation origin

• Mutant Cell Lines: MEPD1008_3_A01, MEPD1008_3_A02, MEPD1008_3_A03, MEPD1008_3_A04, MEPD1008_3_A05, MEPD1008_3_A06, MEPD1008_3_A09, MEPD1008_3_A11, MEPD1008_3_A12, MEPD1008_3_B01, MEPD1008_3_B02, MEPD1008_3_B03, MEPD1008_3_B04, MEPD1008_3_B05, MEPD1008_3_B06, MEPD1008_3_B07, MEPD1008_3_B08, MEPD1008_3_B09, MEPD1008_3_B10, MEPD1008_3_B11, MEPD1008_3_B12, MEPD1008_3_C03, MEPD1008_3_C04, MEPD1008_3_C05, MEPD1008_3_C06, MEPD1008_3_C07, MEPD1008_3_C08, MEPD1008_3_C09, MEPD1008_3_C10, MEPD1008_3_C11, MEPD1008_3_C12, MEPD1008_3_D01, MEPD1008_3_D03, MEPD1008_3_D04, MEPD1008_3_D05, MEPD1008_3_D07, MEPD1008_3_D08, MEPD1008_3_D09, MEPD1008_3_E02, MEPD1008_3_E03, MEPD1008_3_E06, MEPD1008_3_E07, MEPD1008_3_E08, MEPD1008_3_E09, MEPD1008_3_E10, MEPD1008_3_E11, MEPD1008_3_E12, MEPD1008_3_F01, MEPD1008_3_F04, MEPD1008_3_F07, MEPD1008_3_F08, MEPD1008_3_F09, MEPD1008_3_F11, MEPD1008_3_F12, MEPD1008_3_G01, MEPD1008_3_G02, MEPD1008_3_G12, MEPD1008_3_H01, MEPD1008_3_H02, MEPD1008_3_H03, MEPD1008_3_H04, MEPD1008_3_H05, MEPD1008_3_H06, MEPD1008_3_H07, MEPD1008_3_H08, MEPD1008_3_H09, MEPD1008_3_H10, MEPD1008_3_H11
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 90726567 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 90727327. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cfap298 Mutation: 16 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)