Tubb4a^{tm2a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Tubb4a^{tm2a(EUCOMM)Hmgu}
• Name: tubulin, beta 4A class IVA; targeted mutation 2a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5521858
• Gene: Tubb4a Location: Chr17:57387061-57394600 bp, - strand Genetic Position: Chr17, 29.66 cM
• IMPC: Tubb4a gene page

Mutation origin

• Mutant Cell Lines: HEPD0934_4_A01, HEPD0934_4_A03, HEPD0934_4_A05, HEPD0934_4_A06, HEPD0934_4_B04, HEPD0934_4_C05, HEPD0934_4_C07, HEPD0934_4_D01, HEPD0934_4_E02, HEPD0934_4_E05, HEPD0934_4_E07, HEPD0934_4_F02, HEPD0934_4_F04, HEPD0934_4_F05, HEPD0934_4_F06, HEPD0934_4_F08, HEPD0934_4_G03, HEPD0934_4_G08, HEPD0934_4_H01, HEPD0934_4_H07
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_GT0_LF2A_LacZ_BetactP_neo
• Mutation details: The L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 57392855 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 57393590. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tubb4a Mutation: 26 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)