About   Help   FAQ
Targeted Allele Detail
Symbol: Prkcitm2.1(Prkcz)Ltg
Name: protein kinase C, iota; targeted mutation 2.1, Michael Leitges
MGI ID: MGI:5520925
Synonyms: PKCiota/lambda Res
Gene: Prkci  Location: Chr3:30995742-31052740 bp, + strand  Genetic Position: Chr3, 14.65 cM
Growth retardation of E9.5 Prkcitm2.1(Prkcz)Ltg/Prkcitm2.1(Prkcz)Ltg embryos

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:202124
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe cDNA encoding Prkcz followed by a floxed neomycin resistance cassette was inserted into the coding sequence of exon 2. Cre-mediated recombination removed the neomycin resistance cassette. This allele expresses a fusion protein consisting of the first 28 amino acids of the endogenous sequence and the remaining 564 amino acids of the knocked-in sequence. Western blot analysis confirmed the absence of endogenous protein expression and expression of the knock-in sequence in E9.5 embryos. (J:202124)
Generation of the Prkcitm2.1(Prkcz)Ltg allele
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Prkci Mutation:  54 strains or lines available
Original:  J:202124 Seidl S, et al., Phenotypical analysis of atypical PKCs in vivo function display a compensatory system at mouse embryonic day 7.5. PLoS One. 2013;8(5):e62756
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.14
The Jackson Laboratory