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Prkcitm2.1(Prkcz)Ltg
Targeted Allele Detail
Nomenclature
Symbol: Prkcitm2.1(Prkcz)Ltg
Name: protein kinase C, iota; targeted mutation 2.1, Michael Leitges
MGI ID: MGI:5520925
Synonyms: PKCiota/lambda Res
Gene: Prkci  Location: Chr3:30995742-31052740 bp, + strand  Genetic Position: Chr3, 14.65 cM
Growth retardation of E9.5 Prkcitm2.1(Prkcz)Ltg/Prkcitm2.1(Prkcz)Ltg embryos

Show the 1 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:202124
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe cDNA encoding Prkcz followed by a floxed neomycin resistance cassette was inserted into the coding sequence of exon 2. Cre-mediated recombination removed the neomycin resistance cassette. This allele expresses a fusion protein consisting of the first 28 amino acids of the endogenous sequence and the remaining 564 amino acids of the knocked-in sequence. Western blot analysis confirmed the absence of endogenous protein expression and expression of the knock-in sequence in E9.5 embryos. (J:202124)
Generation of the Prkcitm2.1(Prkcz)Ltg allele
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Prkci Mutation:  54 strains or lines available
References
Original:  J:202124 Seidl S, et al., Phenotypical analysis of atypical PKCs in vivo function display a compensatory system at mouse embryonic day 7.5. PLoS One. 2013;8(5):e62756
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/15/2019
MGI 6.14
The Jackson Laboratory