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Bclaf1tm1b(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Bclaf1tm1b(EUCOMM)Wtsi
Name: BCL2-associated transcription factor 1; targeted mutation 1b, Wellcome Trust Sanger Institute
MGI ID: MGI:5520175
Gene: Bclaf1  Location: Chr10:20310512-20342644 bp, + strand  Genetic Position: Chr10, 9.75 cM, cytoband A3
Mutation
origin
Mutant Cell Line:  EPD0599_4_C06
Germline Transmission:  Earliest citation of germline transmission: J:188991
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 20324819 of Chromosome 10 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 20326303. The critical exon(s) were thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding a knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:155845, J:173534, J:188991)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Bclaf1 Mutation:  24 strains or lines available
References
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2020
MGI 6.15
The Jackson Laboratory