Pax7^tm2Tajb
Targeted Allele Detail

Summary

• Symbol: Pax7^tm2Tajb
• Name: paired box 7; targeted mutation 2, Shahragim Tajbakhsh
• MGI ID: MGI:5517378
• Synonyms: Pax7^PN-H2BGFP
• Gene: Pax7 Location: Chr4:139464373-139560839 bp, - strand Genetic Position: Chr4, 70.83 cM
• Alliance: Pax7^tm2Tajb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200727
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The construct replaced the sequence around the transcription initiation site with a loxP site, a Pax7 cDNA (Pax7d) with a polyadenylation sequence, FRT site, neomycin resistance cassette with polyadenylation sequence, loxP site, a GFP gene and mouse chromosome localizing histone H2B fusion (H2BGFP) with a polyadenylation sequence, and an FRT site. The Pax7 cDNA failed to express. (J:200727)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax7 Mutation: 40 strains or lines available

References

• Original: J:200727 Sambasivan R, et al., Embryonic founders of adult muscle stem cells are primed by the determination gene Mrf4. Dev Biol. 2013 Sep 1;381(1):241-55
• All: 1 reference(s)