Hand2^tm2Zllr
Targeted Allele Detail

Summary

• Symbol: Hand2^tm2Zllr
• Name: heart and neural crest derivatives expressed 2; targeted mutation 2, Rolf Zeller
• MGI ID: MGI:5515377
• Gene: Hand2 Location: Chr8:57774018-57777552 bp, + strand Genetic Position: Chr8, 29.8 cM
• Alliance: Hand2^tm2Zllr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201720
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (RMCE-ready)
• Mutation: Insertion
• Mutation details: Double recombinase mediated cassette exchange (dRMCE) was used to replace exon 1 of the endogenous gene with a flag epitope tagged exon1. An unmodified exon 2 replaced endogenous exon 2 and a attB/attP flanked PGK-hygro selection cassette was inserted at the same time. Modification was carried out in Hand2 ES cells in which exons 1 and 2 were flanked by loxP sites and an FRT flanked neomycin cassette was inserted downstream. Replacement was accomplished by simultaneous cre and flp recombinase expression in the ES cells. (J:201720)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hand2 Mutation: 12 strains or lines available

References

• Original: J:201720 Osterwalder M, et al., Dual RMCE for efficient re-engineering of mouse mutant alleles. Nat Methods. 2010 Nov;7(11):893-5
• All: 1 reference(s)