Pold2^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Pold2^{tm1a(EUCOMM)Hmgu}
• Name: polymerase (DNA directed), delta 2, regulatory subunit; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5514576
• Gene: Pold2 Location: Chr11:5822180-5828256 bp, - strand Genetic Position: Chr11, 3.88 cM, cytoband A2
• IMPC: Pold2 gene page

Mutation origin

• Mutant Cell Lines: HEPD0911_5_A04, HEPD0911_5_A09, HEPD0911_5_B06, HEPD0911_5_B07, HEPD0911_5_D05, HEPD0911_5_F10, HEPD0911_5_G02, HEPD0911_5_G05, HEPD0911_5_H11
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_GT1_LF2A_LacZ_BetactP_neo
• Mutation details: The L1L2_GT1_LF2A_LacZ_BetactP_neo cassette was inserted at position 5823862 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 5825978. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pold2 Mutation: 29 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)