Sypl1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sypl1^{tm1b(KOMP)Wtsi}
• Name: synaptophysin like 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5513828
• Synonyms: Sypl1 KO
• Gene: Sypl1 Location: Chr12:33003874-33029503 bp, + strand Genetic Position: Chr12, 13.87 cM, cytoband B2
• Alliance: Sypl1^{tm1b(KOMP)Wtsi} page
• IMPC: Sypl1 gene page

Mutation origin

• Mutant Cell Line: EPD0112_4_C02
• Germline Transmission: Earliest citation of germline transmission: J:200814
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Cre excision of the Sypl1^{tm1a(KOMP)Wtsi} allele removed the promoter-driven neo and critical exon 3, leaving behind the lacZ reporter. Western blotting and immunofluorescence confirmed the absence of encoded protein in homozygous mutant testes. (J:200814, J:339267)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sypl1 Mutation: 16 strains or lines available

References

• Original: J:200814 Toronto Centre for Phenogenomics, Strains and alleles submitted by Toronto Centre for Phenogenomics (NorCOMM2, funded by Genome Canada and Ontario Genomics Institute OGI-051). MGI Direct Data Submission. 2013
• All: 4 reference(s)