Pik3c2a^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Pik3c2a^{tm1c(EUCOMM)Hmgu}
• Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5513808
• Gene: Pik3c2a Location: Chr7:115936500-116042684 bp, - strand Genetic Position: Chr7, 61.62 cM
• Alliance: Pik3c2a^{tm1c(EUCOMM)Hmgu} page
• IMPC: Pik3c2a gene page

Mutation origin

• Mutant Cell Line: HEPD0636_4_G04
• Germline Transmission: Earliest citation of germline transmission: J:200814
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 116004870 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 116005664. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
  The exon flanked by loxP sites in this allele is the exon following the exon containing the ATG translation start site. (The nomenclature of the mouse Pik3c2a gene exons has been inconsistent in the published literature. (J:370019) (J:200814, J:370019)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pik3c2a Mutation: 99 strains or lines available

References

• Original: J:200814 Toronto Centre for Phenogenomics, Strains and alleles submitted by Toronto Centre for Phenogenomics (NorCOMM2, funded by Genome Canada and Ontario Genomics Institute OGI-051). MGI Direct Data Submission. 2013
• All: 3 reference(s)