Dync1i1^tm1Geno
Targeted Allele Detail

Summary

• Symbol: Dync1i1^tm1Geno
• Name: dynein cytoplasmic 1 intermediate chain 1; targeted mutation 1, Genoway
• MGI ID: MGI:5512904
• Synonyms: IC-1-GFP-3xFLAG
• Gene: Dync1i1 Location: Chr6:5725772-6028030 bp, + strand Genetic Position: Chr6, 2.26 cM
• Alliance: Dync1i1^tm1Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201230
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 17. An EGFP and 3 x FLAG tag was inserted into exon 17 upstream of the stop codon. Flp-mediated recombination removed the neomycin resistance cassette. Western blot analysis confirmed the presence of the fusion protein. (J:201230)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dync1i1 Mutation: 31 strains or lines available

References

• Original: J:201230 Zhang J, et al., Establishing a novel knock-in mouse line for studying neuronal cytoplasmic dynein under normal and pathologic conditions. Cytoskeleton (Hoboken). 2013 Apr;70(4):215-27
• All: 3 reference(s)