Tg(Vpreb1-IL2RB)F133Ilm
Transgene Detail

Summary

• Symbol: Tg(Vpreb1-IL2RB)F133Ilm
• Name: transgene insertion F133, Inga-Lill Martensson
• MGI ID: MGI:5505492
• Synonyms: -214BpreB1-huCD122 line F133, Tg(-214VpreB1-huCD122)F133Lmb, Tg(214VpreB1-huCD122)F133mb
• Transgene: Tg(Vpreb1-IL2RB)F133Ilm Location: unknown
• Alliance: Tg(Vpreb1-IL2RB)F133Ilm page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Vpreb1-IL2RB)F133Ilm expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IL2RB (3560)

  Il2rb

  (MGI:96550)

• Mutation details: This transgenic insertion comprises 10 copies, as determined by dot blot and Southern blot analysis, of a 3.7-kb construct containing the mouse pre-B lymphocyte gene 1 (Vprb1) promoter and enhancer (nt -214 to +142, counting the 5'-most transcriptional initiation site as +1) driving expression of the human interleukin 2 receptor, beta (IL2RB, also called CD122) cDNA, which is followed by exon 2, intron 2, exon 3 and the polyadenylation signal of the human beta-globin gene. Semi-quantitative reverse transcription- (RT-) PCR analysis reveals transgene expression at high levels in bone marrow (BM) and thymus and low to very low levels in spleen, brain and muscle; signal is undetectable in liver. Similar analysis of sorted BM cells from mice of this line reveals expression in the B220⁺Igm^- pro-/pre-B cell, but not in the B220⁺Igm⁺ immature/mature B cell population, concordant with the expression pattern of native Vpreb1. Flow cytometric analysis demonstrates that the human IL2RB marker is present on ~10% of B220^low/int (characteristic of pro-/pre- and immature B) cells, but not on B220^high (mature B) cells nor on B220^- cells. (J:199513)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:199513 Licence S, et al., The VpreB1 enhancer drives developmental stage-specific gene expression in vivo. Eur J Immunol. 2003 Apr;33(4):1117-26
• All: 1 reference(s)