Gt(ROSA)26Sor^{tm1(CAG-tdTomato*,-EGFP*)Ees}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-tdTomato*,-EGFP*)Ees}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Edward E Schmidt
• MGI ID: MGI:5504463
• Synonyms: Gt(ROSA)26Sor^{tm1(ACTB-tdTomato*,-EGFP*)Ees}, Rosa26^nTnG, ROSA^nT-nG
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-tdTomato*,-EGFP*)Ees} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201735
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The nT-nG targeting vector was made by modifying the Rosa26 mT/mG plasmid (see JAX Stock No. 007676). Modifications replaced the N-terminal membrane-targeting domains of tdTomato and EGFP with strong start codons, and added sequences at the C-termini of tdTomato and EGFP that encode amino acids 300-350 of the SRm160 nuclear assembly domain (to direct the stable integration of each fluorescent protein into the nucleus). The final nT-nG targeting vector contained (from 5' to 3') a CMV enhancer/chicken beta-actin core promoter (pCA), a loxP site, the nT cassette (tdTomato protein sequence [non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)] with C-terminal SRm160 nuclear targeting signal and a polyadenylation signal), a second loxP site, the nG cassette (an enhanced green fluorescent protein [EGFP] sequence with C-terminal SRm160 nuclear targeting signal and a polyadenylation signal), and an frt-flanked neo cassette. The SRm160 sequences attached to tdTomato and EGFP contain different synonymous mutations (and the SRm160 sequence in tdTomato also has a single non-synonymous mutation) to avoid potential homologous recombination in bacteria between the SRm160 sequences in the nT cassette and nG cassette. (J:199711, J:201735)

Expression

In Mice Carrying this Mutation:

13 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:201735 Prigge JR, et al., Nuclear double-fluorescent reporter for in vivo and ex vivo analyses of biological transitions in mouse nuclei. Mamm Genome. 2013 Sep 11
• All: 45 reference(s)