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Targeted Allele Detail
Symbol: Pou5f1tm1Grc
Name: POU domain, class 5, transcription factor 1; targeted mutation 1, Gerald R Crabtree
MGI ID: MGI:5504452
Gene: Pou5f1  Location: Chr17:35506018-35510772 bp, + strand  Genetic Position: Chr17, 18.69 cM
Germline Transmission:  Earliest citation of germline transmission: J:199710
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA BAC containing the mouse locus was manipulated through recombineering. The construct was flanked with homology arms of approximately 3.7 kbp upstream and 10.9 kbp downstream. Two distinct DNA binding sequence arrays were added at -277 bp upstream of the transcriptional start of of the gene, 12 x ZFHD1 (TAATGATGGGCG) and 5 x Gal4 (CGGAGTACTGTCCTCCGAG). A nuclear EGFP was inserted at the ATG of exon 1, exons 2-5 were deleted after insertion to avoid expression of endogenous Pou5f1 from the reporter allele. A floxed neomycin resistance cassette was inserted behind the EGFP reporter for positive selection and thymidine kinase was inserted behind the homology arms for negative selection. (J:199709, J:199710)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pou5f1 Mutation:  75 strains or lines available
Original:  J:199710 Crabtree GR, Direct Data Submission for Pou5fl. MGI Direct Data Submission. 2013;
All:  2 reference(s)

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