Spi1^tm4.1Dgt
Targeted Allele Detail

Summary

• Symbol: Spi1^tm4.1Dgt
• Name: Spi-1 proto-oncogene; targeted mutation 4.1, Daniel G Tenen
• MGI ID: MGI:5501529
• Synonyms: PU.1^ki
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm4.1Dgt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196609
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector introduced the floxed nucleotide substitution of GGAA to TCGC in the 14 kb upstream regulatory element (URE) at the PU.1 site. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted upstream of the modified region. Real-time PCR confirmed reduced transcript expression. This allele is hypomorphic. (J:196609)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 27 strains or lines available

References

• Original: J:196609 Staber PB, et al., Sustained PU.1 levels balance cell-cycle regulators to prevent exhaustion of adult hematopoietic stem cells. Mol Cell. 2013 Mar 7;49(5):934-46
• All: 2 reference(s)