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Pvalbtm5.1(cre/folA)Hze
Targeted Allele Detail
Nomenclature
Symbol: Pvalbtm5.1(cre/folA)Hze
Name: parvalbumin; targeted mutation 5.1, Hongkui Zeng
MGI ID: MGI:5501499
Synonyms: Pvalb-2A-dCre, Pvalb-2A-dCre-DeltaHygrO, Pvalb-2A-hDHFR/Cre-D, Pvalb-2A-hDHFR/Cre-DeltaHygro
Gene: Pvalb  Location: Chr15:78191114-78206400 bp, - strand  Genetic Position: Chr15, 36.93 cM, cytoband E
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:199219
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    trimethoprim
Mutation:    Insertion
 
Mutation detailsES cells previously targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon were re-targeted with a "T2A-hDHFR/Cre" vector and a FLP-expressing plasmid to facilitate recombination. Correctly re-targeted ES cells had (from 5' to 3') a partial Pvalb intron 3 sequence containing an frt3 site, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A in-frame with the Pvalb coding sequence, a dCre fusion gene that is in-frame with the Pvalb codingsequence, the Pvalb 3' UTR sequence from exon 4, an AttB site, a PGK-hygromycin-SV40polyA cassette (with an mRNA spice donor-frt5 site-mRNA spice acceptor in the hygromycin gene), and an AttP site. The dCre fusion gene ( destabilized Cre, hDHFR/Cre or ecDHFR/Cre) is Cre recombinase fused at its N terminus to the first 159 amino acids of the E.coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) with the G67S mutation and modified to include the R12Y/Y100I destabilizing domain mutations. Correctly re-targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace them with the recombined AttB/AttP site (AttL). With trimethoprim induction, cre expression (by ISH detection) is efficient in scattered cells throughout the cortex, as well as restricted populations in the cerebellum, medulla, pons, pallidum, and thalamus. (J:199219, J:220523)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Pvalb     Summary of all recombinase alleles driven by Pvalb. Comparative matrix view of recombinase activities
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pvalb Mutation:  24 strains or lines available
References
Original:  J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/24/2020
MGI 6.15
The Jackson Laboratory