Pvalb^{tm5.1(cre/folA)Hze}
Targeted Allele Detail

Summary

• Symbol: Pvalb^{tm5.1(cre/folA)Hze}
• Name: parvalbumin; targeted mutation 5.1, Hongkui Zeng
• MGI ID: MGI:5501499
• Synonyms: Pvalb-2A-dCre, Pvalb-2A-dCre-DeltaHygrO, Pvalb-2A-hDHFR/Cre-D, Pvalb-2A-hDHFR/Cre-DeltaHygro
• Gene: Pvalb Location: Chr15:78075314-78090600 bp, - strand Genetic Position: Chr15, 36.93 cM, cytoband E
• Alliance: Pvalb^{tm5.1(cre/folA)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199219
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: trimethoprim
• Mutation: Insertion
• Pvalb^{tm5.1(cre/folA)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pvalb (MGI:97821)

• Mutation details: ES cells previously targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon were re-targeted with a "T2A-hDHFR/Cre" vector and a FLP-expressing plasmid to facilitate recombination. Correctly re-targeted ES cells had (from 5' to 3') a partial Pvalb intron 3 sequence containing an frt3 site, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A in-frame with the Pvalb coding sequence, a dCre fusion gene that is in-frame with the Pvalb codingsequence, the Pvalb 3' UTR sequence from exon 4, an AttB site, a PGK-hygromycin-SV40polyA cassette (with an mRNA spice donor-frt5 site-mRNA spice acceptor in the hygromycin gene), and an AttP site. The dCre fusion gene ( destabilized Cre, hDHFR/Cre or ecDHFR/Cre) is Cre recombinase fused at its N terminus to the first 159 amino acids of the E.coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) with the G67S mutation and modified to include the R12Y/Y100I destabilizing domain mutations. Correctly re-targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace them with the recombined AttB/AttP site (AttL). With trimethoprim induction, cre expression (by ISH detection) is efficient in scattered cells throughout the cortex, as well as restricted populations in the cerebellum, medulla, pons, pallidum, and thalamus. (J:199219, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pvalb (mouse) Summary of all recombinase alleles driven by Pvalb. Comparative matrix view of recombinase activities Alliance mouse Pvalb gene page

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pvalb Mutation: 29 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 2 reference(s)