Lman1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Lman1^{tm1c(KOMP)Wtsi}
• Name: lectin, mannose-binding, 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5501339
• Synonyms: Lman1^fl
• Gene: Lman1 Location: Chr18:66113810-66135706 bp, - strand Genetic Position: Chr18, 39.08 cM
• Alliance: Lman1^{tm1c(KOMP)Wtsi} page
• IMPC: Lman1 gene page

Mutation origin

• Mutant Cell Line: EPD0807_3_B06
• Germline Transmission: Earliest citation of germline transmission: J:213133
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 66131224 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 66132252. The critical exons 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can is created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:213133)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lman1 Mutation: 21 strains or lines available

Notes

Cell line EPD0807_3_B06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:213133.

References

• Original: J:213133 Everett LA, et al., Murine coagulation factor VIII is synthesized in endothelial cells. Blood. 2014 Jun 12;123(24):3697-705
• All: 2 reference(s)