Gt(ROSA)26Sor^{tm3(CAG-PSTPIP1*)Dtg}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-PSTPIP1*)Dtg}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Douglas T Golenbock
• MGI ID: MGI:5496226
• Synonyms: Rosa-26-PSTPIP1 E250Q^{STOP del}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-PSTPIP1*)Dtg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196002
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3(CAG-PSTPIP1*)Dtg} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PSTPIP1 (9051)

  Pstpip1

  (MGI:1321396)

• Mutation details: The targeting construct inserted into the locus contained a CAG promoter that drives ubiquitous expression of the human cDNA with nucleotide substitutions that result in the amino acid substitution of glutamic acid for aspartic acid at position 250 (E250Q) preceded by a floxed STOP cassette and followed by an IRES and the truncated human CD2 tag. (J:196002)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:196002 Wang D, et al., Inflammation in mice ectopically expressing human Pyogenic Arthritis, Pyoderma Gangrenosum, and Acne (PAPA) Syndrome-associated PSTPIP1 A230T mutant proteins. J Biol Chem. 2013 Feb 15;288(7):4594-601
• All: 1 reference(s)