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Transgene Detail
Symbol: Tg(tetO-MAML1*/EGFP)2Akar
Name: transgene insertion 2, Aly Karsan
MGI ID: MGI:5496022
Synonyms: dnMAML-GFP, TetOS-dnMAML, TetOS-dnMAML-GFP
Transgene: Tg(tetO-MAML1*/EGFP)2Akar  Location: unknown  
Strain of Origin:  ICR
Transgene Type:    Transgenic (Dominant negative, Inducible, Inserted expressed sequence, Reporter)
Mutation:    Insertion
Tg(tetO-MAML1*/EGFP)2Akar expresses 1 gene
Mutation detailsThe Tet-dnMAML-GFP transgene was designed with a TetOS promoter followed by a cDNA sequence encoding the dnMAML-GFP fusion protein and an SV40 polyA sequence. The TetOS promoter (Tet response element or TRE) contains seven copies of the 42-bp tet operator sequence (tetO) just upstream of a minimal CMV promoter. The dnMAML-GFP cDNA sequence encodes an N-terminal, 62 amino acid (amino acids 13-74), dominant-negative mutant variant of human Mastermind-like 1 (dnMAML) fused in-frame at its C-terminus to an enhanced green fluorescent protein (EGFP). (J:183913)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:183913 Chang L, et al., Differentiation of vascular smooth muscle cells from local precursors during embryonic and adult arteriogenesis requires Notch signaling. Proc Natl Acad Sci U S A. 2012 May 1;109(18):6993-8
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.15
The Jackson Laboratory