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Targeted Allele Detail
Symbol: Dlg4tm1.1Tdoi
Name: discs large MAGUK scaffold protein 4; targeted mutation 1.1, Tomoko Doi
MGI ID: MGI:5495768
Synonyms: 1d2d-PSD-95-EGFP
Gene: Dlg4  Location: Chr11:70017203-70045535 bp, + strand  Genetic Position: Chr11, 42.96 cM, cytoband B4
Germline Transmission:  Earliest citation of germline transmission: J:197875
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsExons 5 through 8 were replaced with a modified full-length cDNA and EGFP fusion followed by a floxed neomycin resistance cassette. The modified cDNA contains the mutations S78N (AGC to AAC), A80V (GCA to GTA), S183N (AGC to AAC), and A185V (GCA to GTA), and the deletions T83 to I88 and V178 to I183 that mutate PDZ1/2 to resemble PDZ3. Cre-mediated recombination removed the neomycin resistance cassette. (J:197875)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Dlg4 Mutation:  25 strains or lines available
Original:  J:197875 Nagura H, et al., Impaired synaptic clustering of postsynaptic density proteins and altered signal transmission in hippocampal neurons, and disrupted learning behavior in PDZ1 and PDZ2 ligand binding-deficient PSD-95 knockin mice. Mol Brain. 2012;5:43
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory