Tg(Nr5a1/EGFP)1Klp
Transgene Detail

Summary

• Symbol: Tg(Nr5a1/EGFP)1Klp
• Name: transgene insertion 1, Keith L Parker
• MGI ID: MGI:5493455
• Synonyms: Sf1-eGFP, Sf1:eGFP, Sf1;eGFP, SF-1/eGFP transgene
• Transgene: Tg(Nr5a1/EGFP)1Klp Location: unknown
• Alliance: Tg(Nr5a1/EGFP)1Klp page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Nr5a1/EGFP)1Klp expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nr5a1 (MGI:1346833)

• Mutation details: This transgene contains a 50-kb bacterial artificial chromosome- (BAC-) derived mouse genomic DNA fragment including ~45 kb of 5' flanking sequence from the mouse Nr5a1 gene, the noncoding first exon, the first intron, and 32 nucleotides of the second exon followed by the coding sequence for enhanced green fluorescent protein (EGFP) and the 3' splicing and polyadenylation signals of the bovine growth hormone gene. The expressed protein comprises the first 5 amino acids of mouse NR5A1 and an alanine joined to EGFP at its initiating methionine. Fluorescent and immunohistochemical analysis for EGFP demonstrate transgene expression in steroidogenic cells of the adrenal cortex, testes and ovaries, in neurons of the ventromedial hypothalamic nucleus, and in reticuloendothelial cells of the spleen; EGFP is not expressed in the anterior pituitary. (J:197600)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:197600 Stallings NR, et al., Development of a transgenic green fluorescent protein lineage marker for steroidogenic factor 1. Mol Endocrinol. 2002 Oct;16(10):2360-70
• All: 13 reference(s)