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Targeted Allele Detail
Symbol: Igs6tm2.1(ACTB-EFGP,-tdTomato)Luo
Name: intergenic site 6; targeted mutation 2.1, Liqun Luo
MGI ID: MGI:5491599
Synonyms: Iis6tm2.1(ACTB-EFGP,-tdTomato)Luo, John12GT, John12-GT, M12-GT, MADM-12GT, MADM-12GT, MADM-12-GT, MADM-12^TG
Gene: Igs6  Location: unknown  Genetic Position: Chr12, 1.71 cM
Germline Transmission:  Earliest citation of germline transmission: J:194079
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
Mutation detailsA targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "GT MADM" cassette. The "GT MADM" cassette used here was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire "GT MADM" construct was inserted into the John12 locus on chromosome 12 (~1.71 cM; ~16 kbp downstream of exon 1 of the Rab10 gene). Cre-mediated recombination removed the floxed neo cassette. (J:194079)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igs6 Mutation:  2 strains or lines available
Original:  J:194079 Hippenmeyer S, et al., Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance. Cell Rep. 2013 Mar 28;3(3):960-7
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory