Tg(Pten)GPpp
Transgene Detail

Summary

• Symbol: Tg(Pten)GPpp
• Name: transgene insertion G, Pier Paolo Pandolfi
• MGI ID: MGI:5491064
• Synonyms: BAC-PTEN TG, BAC-PTEN TG line 3, Super-PTEN, Super-PTEN line 3, Tg(RP23-215F15)GPpp
• Transgene: Tg(Pten)GPpp Location: unknown
• Alliance: Tg(Pten)GPpp page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pten)GPpp expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pten (MGI:109583)

• Mutation details: The transgene comprises the 218.50-kb mouse genomic DNA insert of the bacterial artificial chromosome (BAC) clone RP23-215F15 (RPCI library; C57BL/6J), which contains the entire phosphatase and tensin homolog (Pten) gene. Immunoblot analysis of whole-cell protein extracts from mouse embryonic fibroblasts (MEFs) cultured from E13.5 embryos of this line shows that they express the protein at 3.5-fold the level of wild-type MEFs; elevated levels are observed in both cytoplasmic and nuclear MEF extracts. Elevated PTEN protein expression is observed in embryos and in all adult tissues analyzed: brain, lung, kidney, heart, thymus, liver, spleen, and white and brown adipose tissue. (J:186096)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:186096 Garcia-Cao I, et al., Systemic elevation of PTEN induces a tumor-suppressive metabolic state. Cell. 2012 Mar 30;149(1):49-62
• All: 2 reference(s)