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Transgene Detail
Symbol: Tg(Prnp*P101L)174Sbp
Name: transgene insertion 174, Stanley B Prusiner
MGI ID: MGI:5487544
Synonyms: Tg(GSSPrP)174, Tg(MoPrP-P101L)174
Transgene: Tg(Prnp*P101L)174Sbp  Location: unknown  
Strain of Origin:  (C57BL/6 x SJL)F2
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Prnp*P101L)174Sbp expresses 1 gene
Mutation detailsThis transgene consists of the murine Prnp with a proline to leucine substitution at codon 101 (equivalent to codon 102 in human PRNP). This construct was generated using a chimeric murine cosmid constructed by exchanging the BamHI-SalI fragment containing the open reading frame from a recombinant NZW genomic clone for the corresponding fragment in an ILn/J mouse cosmid. An approximate 100-nucleotide region flanking codon 101 leucine was modified to yield DNA sequence homology to hamster Prnp while retaining amino acid homology to mouse Prnp. Analysis indicated that this line carries approximately 64 haploid transgene copies inserted into a single autosomal site. Three lines were created: 174, 180, and 196. (J:127373)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 1 anatomical structures
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Original:  J:127373 Hsiao KK, et al., Spontaneous neurodegeneration in transgenic mice with mutant prion protein. Science. 1990 Dec 14;250(4987):1587-90
All:  1 reference(s)

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MGI 6.13
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