Tg(Gad1-cre,-lacZ)1Mini
Transgene Detail

Summary

• Symbol: Tg(Gad1-cre,-lacZ)1Mini
• Name: transgene insertion, Liliana Minichiello
• MGI ID: MGI:5486478
• Synonyms: Gad1-Cre, GAD67-Cre
• Transgene: Tg(Gad1-cre,-lacZ)1Mini Location: unknown
• Alliance: Tg(Gad1-cre,-lacZ)1Mini page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Gad1-cre,-lacZ)1Mini expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gad1 (MGI:95632)

• Mutation details: A cassette consisting of a hybrid intron, the cre-IRES-lacZ sequence and a downstream frt-flanked neo selection marker (in reverse orientation) was inserted into the first coding ATG of the Gad1 locus in a BAC clone (RPC123-118N13) by ET recombination in bacteria. After selection of correctly recombined cells, Flp expression excised the neo cassette. The circular BAC DNA construct was injected into pronuclei of oocytes. Offspring of founder mice were tested by X-gal staining for presence of a functional lacZ cassette. Staining was weak possibly due to low efficiency of the IRES site. Cre mediated EYFP or EGFP expression was used to assess recombination in crosses with reporter mice. (J:195054)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gad1 (mouse) Summary of all recombinase alleles driven by Gad1. Alliance mouse Gad1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:195054 Ohtsuka N, et al., GABAergic neurons regulate lateral ventricular development via transcription factor Pax5. Genesis. 2013 Apr;51(4):234-45
• All: 3 reference(s)