Serpinb5^tm1.1Pib
Targeted Allele Detail

Summary

• Symbol: Serpinb5^tm1.1Pib
• Name: serine (or cysteine) peptidase inhibitor, clade B, member 5; targeted mutation 1.1, Phillip I Bird
• MGI ID: MGI:5484764
• Gene: Serpinb5 Location: Chr1:106788905-106811078 bp, + strand Genetic Position: Chr1, 50.34 cM
• Alliance: Serpinb5^tm1.1Pib page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206439
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP site was into intron 3. An FRT-flanked neo with a 3' loxP was into intron 4. Cre-mediated recombination removed the neo cassette and exon 4. RNA analysis of mammary tissue form homozygous mice detects only a mutant truncated RNA product. Immunoblot and immunohistochemical analyses confirmed the absence of protein in epidermis from homozygous mice. Depending on the antibody used a 42kDa species is detected in the thymus and prostate; however, this is thought to be the result of cross-reaction with of clade B serpins. (J:206439)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Serpinb5 Mutation: 34 strains or lines available

References

• Original: J:206439 Teoh SS, et al., Maspin is not required for embryonic development or tumour suppression. Nat Commun. 2014;5:3164
• All: 2 reference(s)