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Tac1tm1.1(cre)Hze
Targeted Allele Detail
Nomenclature
Symbol: Tac1tm1.1(cre)Hze
Name: tachykinin 1; targeted mutation 1.1, Hongkui Zeng
MGI ID: MGI:5484636
Synonyms: Tac1-IRES2-Cre, Tac1-IRES2-Cre-Delta, Tac1-IRES2-Cre-DeltaNeo/Hygro, Tac1-IRES2-Cre-DeltaNeo/HygroTac1-IRES2-Cre-D
Gene: Tac1  Location: Chr6:7555061-7562978 bp, + strand  Genetic Position: Chr6, 3.31 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:195475
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsThe targeting vector contained, from 5' to 3', a partial Tac1 sequence spanning intron 4 through intron 6, an frt3 site within Tac1 intron 6, a partial Tac1 exon 7 sequence including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of a mRNA sequence), a cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice (JAX Stock No. 007743) to remove the AttB/AttP-flanked sequences. (J:195475, J:220523)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Tac1     Summary of all recombinase alleles driven by Tac1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tac1 Mutation:  33 strains or lines available
References
Original:  J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:  25 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory