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Snap25tm1.1Hze
Targeted Allele Detail
Nomenclature
Symbol: Snap25tm1.1Hze
Name: synaptosomal-associated protein 25; targeted mutation 1.1, Hongkui Zeng
MGI ID: MGI:5484607
Synonyms: Ai52, Ai52D, Snap25-2A, Snap25-LSL-2A-EGFP, Snap25-LSL-2A-EGFP-Delta, Snap25-LSL-2A-EGFP-DeltaNeo/Hygro, Snap25tm1.1(xstpx-EGFP)Hze
Gene: Snap25  Location: Chr2:136555373-136624348 bp, + strand  Genetic Position: Chr2, 67.56 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsThe targeting vector contained, from 5' to 3', a partial Snap25 sequence spanning intron 6 through intron 7, an frt3 site within Snap25 intron 7, a partial Snap25 exon 8 sequence up to (but not including) the endogenous stop codon, a loxP-flanked STOP cassette that is in-frame with the upstream Snap25 exon 8 sequence (contains translational stop codons in all three reading frames and the PGK polyA sequence), an in-frame F2A peptide cleavage signal, an in frame EGFP gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). (J:101977, J:219930)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Snap25 Mutation:  24 strains or lines available
References
Original:  J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory