Foxo1^tm1Miya
Targeted Allele Detail

Summary

• Symbol: Foxo1^tm1Miya
• Name: forkhead box O1; targeted mutation 1, Jun-ichi Miyazaki
• MGI ID: MGI:5477796
• Synonyms: Foxo1^flox
• Gene: Foxo1 Location: Chr3:52175758-52257530 bp, + strand Genetic Position: Chr3, 23.19 cM
• Alliance: Foxo1^tm1Miya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195101
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted usptream of exon 2. An IRES/beta-geo cassette was fused to the coding sequence of exon 2. An additional loxP site was inserted downstream of exon 2. (J:195101)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxo1 Mutation: 31 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a (129P2/OlaHsd). EB3 cells carry one inactive allele of Pou5f1. Line EB3 was created by targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:195101 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:195101 Miyazaki S, et al., Analysis of Foxo1-regulated genes using Foxo1-deficient pancreatic beta cells. Genes Cells. 2012 Sep;17(9):758-67
• All: 5 reference(s)