Tg(UBC-PA-GFP)1Mnz
Transgene Detail

Summary

• Symbol: Tg(UBC-PA-GFP)1Mnz
• Name: transgene insertion 1, Michel C Nussenzweig
• MGI ID: MGI:5476728
• Transgene: Tg(UBC-PA-GFP)1Mnz Location: unknown
• Alliance: Tg(UBC-PA-GFP)1Mnz page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This transgene comprises 1.2 kb of 5'-flanking DNA (nucleotides -1225 to -6) of the human ubiquitin C gene, which is expressed in a broad range of cell types, including all hematopoietic cells, driving expression of a photoactivatable green fluorescent protein cDNA, which is followed by both the SV40 and the bovine growth hormone polyadenylation signals. PA-GFP differs from EGFP by the substitution of histidine for threonine at amino acid position 203 (T203H); this modification results in ~100-fold enhanced fluorescence of PA-GFP upon excitation at 488 nm after preliminary photoactivation with intense 413-nm light, greatly increasing the optical contrast. (J:33045, J:93490, J:166836, J:195187)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:166836 Victora GD, et al., Germinal center dynamics revealed by multiphoton microscopy with a photoactivatable fluorescent reporter. Cell. 2010 Nov 12;143(4):592-605
• All: 22 reference(s)